https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 c-Myc inactivation of p53 through the pan-cancer lncRNA MILIP drives cancer pathogenesis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37757 ARF in human and p19ARF in mouse) that binds to and inhibits mouse double minute 2 homolog (MDM2) leading to p53 activation, whereas p53 suppresses c-Myc through a combination of mechanisms involving transcriptional inactivation and microRNA-mediated repression. Nonetheless, the regulatory interactions between c-Myc and p53 are not retained by cancer cells as is evident from the often-imbalanced expression of c-Myc over wildtype p53. Although p53 repression in cancer cells is frequently associated with the loss of ARF, we disclose here an alternate mechanism whereby c-Myc inactivates p53 through the actions of the c-Myc-Inducible Long noncoding RNA Inactivating P53 (MILIP). MILIP functions to promote p53 polyubiquitination and turnover by reducing p53 SUMOylation through suppressing tripartite-motif family-like 2 (TRIML2). MILIP upregulation is observed amongst diverse cancer types and is shown to support cell survival, division and tumourigenicity. Thus our results uncover an inhibitory axis targeting p53 through a pan-cancer expressed RNA accomplice that links c-Myc to suppression of p53.]]> Wed 17 Nov 2021 16:28:34 AEDT ]]> Dynamic Landscape of Extracellular Vesicle-Associated Proteins Is Related to Treatment Response of Patients with Metastatic Breast Cancer https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40283 Wed 13 Mar 2024 08:47:04 AEDT ]]> Cell lineage tracing identifies hormone-regulated and Wnt-responsive vaginal epithelial stem cells https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38201 Wed 11 Aug 2021 10:05:47 AEST ]]> Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50850 Wed 09 Aug 2023 09:39:07 AEST ]]> Schwann cell stimulation of pancreatic cancer cells: a proteomic analysis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37505 Wed 02 Mar 2022 14:28:59 AEDT ]]> Phospho-heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51137 Tue 22 Aug 2023 15:58:50 AEST ]]> Proteomic profiling of mouse epididymosomes reveals their contributions to post-testicular sperm maturation https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35957 Tue 21 Jan 2020 11:08:08 AEDT ]]> Time-resolved proteomic profiling of cigarette smoke-induced experimental chronic obstructive pulmonary disease https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43823 Tue 04 Oct 2022 11:04:24 AEDT ]]> Development and characterization of human fetal female reproductive tract organoids to understand Müllerian duct anomalies https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53981 Thu 25 Jan 2024 12:57:12 AEDT ]]> Mass Spectrometry Proteomic Analysis of Four p53 Patterns in Differentiated Vulvar Intraepithelial Neoplasia https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49981 Thu 22 Jun 2023 11:59:40 AEST ]]> Oestrogen fuels the growth of endometrial hyperplastic lesions initiated by overactive Wnt/β-catenin signalling https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43322 Thu 15 Sep 2022 14:43:38 AEST ]]> PI3K/mTOR is a therapeutically targetable genetic dependency in diffuse intrinsic pontine glioma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:55040 Thu 04 Apr 2024 13:51:52 AEDT ]]> Proteomic characterization of the extracellular matrix of human uterine fibroids https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41255 Sat 30 Jul 2022 13:01:25 AEST ]]> Extracellular matrix (ECM) activates β-catenin signaling in uterine fibroids https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44716 Fri 21 Oct 2022 09:04:25 AEDT ]]> Prevalence and clinical significance of co-existing mutations in MED12 and FH in uterine fibroids of Australian women https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:53114 Fri 17 Nov 2023 11:41:36 AEDT ]]> Proteomic profiling of human uterine fibroids reveals upregulation of the extracellular matrix protein periostin https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35929 MED12 alterations in 39 of 65 fibroids (60%) from 14 patients. Using isobaric tagged-based quantitative mass spectrometry on three selected patients (n = 9 fibroids), we observed a common set of upregulated ( > 1.5-fold) and downregulated ( < 0.66-fold) proteins in small, medium, and large fibroid samples of annotated MED12 status. These two sets of upregulated and downregulated proteins were the same in all patients, regardless of variations in fibroid size and MED12 status. We then focused on one of the significant upregulated ECM proteins and confirmed the differential expression of periostin using western blotting and immunohistochemical analysis. Our study defined the proteome of uterine fibroids and identified that increased ECM protein expression, in particular periostin, is a hallmark of uterine fibroids regardless of MED12 mutation status. This study sets the foundation for further investigations to analyze the mechanisms regulating ECM overexpression and the functional role of upregulated ECM proteins in leiomyogenesis.]]> Fri 17 Jan 2020 11:35:08 AEDT ]]> Proteomic analysis identifies tenascin-C expression is upregulated in uterine fibroids https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45565 1.5-fold) with a confidence corresponding to false discovery rate (FDR) <1% in small-, medium-, and large-sized fibroid samples regardless of MED12 mutation status. The TNC was validated on additional patient samples using Western blotting (WB) and immunohistochemistry (IHC) and confirmed significant overexpression of this protein in fibroids compared to matched ANM. Proteomic analyses have identified the increased ECM protein expression, TNC, as a hallmark of uterine fibroids regardless of MED12 mutations. Further functional studies focusing on the upregulated ECM proteins in leiomyogenesis will lead to the identification of novel ECM drug targets for fibroid treatment.]]> Fri 04 Nov 2022 14:44:56 AEDT ]]> Bovine and human endometrium-derived hydrogels support organoid culture from healthy and cancerous tissues https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51354 Fri 01 Sep 2023 13:44:28 AEST ]]> Loss of liver kinase B1 in human seminoma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51353 Fri 01 Sep 2023 13:35:36 AEST ]]>